Journal: Bioactive Materials

Article Title: Osteoporosis treatment via programmed delivery of IRF5 siRNA to macrophages in bone tissue combined with ROS scavenging

doi: 10.1016/j.bioactmat.2026.10.015

Figure Lengend Snippet: siP@WM normalizes the high bone turnover state in ovariectomy-induced osteoporotic mice. (a) Confocal fluorescence images showing nanoparticle-associated fluorescence (red dots) in undecalcified bone sections after 4 weeks of treatment; (b) Osteoclast activity in femora after treatment, shown as (i) representative tartrate-resistant acid phosphatase (TRAP) staining images of decalcified femur sections and (ii) quantification of TRAP + cell number per mm 2 ; (c) Serum levels of bone turnover markers, including C-terminal telopeptide of type I collagen (CTX-Ⅰ) and procollagen type I N-terminal propeptide (P1NP), measured by ELISA; (d) Dynamic histomorphometric analysis of bone formation by sequential fluorescent labeling, shown as (i) quantification of mineral apposition and (ii) representative fluorescence images of femur sections. Green: calcein; yellow: tetracycline. Data are presented as mean ± SD (n = 3 mice per group). Statistical significance was determined by one-way ANOVA followed by Tukey's multiple-comparisons test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; ns, not significant.

Article Snippet: In order to assess potential effects of nanoparticles on bone metabolism, serum levels of mouse bone turnover markers, including procollagen type I N-terminal propeptide (P1NP, Cusabio) and C-terminal telopeptide of type I collagen (CTX-I, Elabscience), were measured using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturers’ protocols.

Techniques: Confocal, Fluorescence, Activity Assay, Staining, Enzyme-linked Immunosorbent Assay, Analysis, Labeling

Journal: Molecular Medicine

Article Title: CD5L insufficiency exacerbates skeletal joint damage in rheumatoid arthritis

doi: 10.1186/s10020-026-01456-x

Figure Lengend Snippet: Immune profile of WT and CD5L⁻ mice during CIA. A Serum CD5L levels measured in WT mice after immunization. p -values were obtained using the Kruskal–Wallis test with Dunn’s multiple comparisons. B Frequencies of blood leukocyte subsets in WT ( n = 10) and CD5L⁻ ( n = 10) mice. Leukocytes were phenotyped by flow cytometry: CD11b + cells, monocytes (Siglec-F⁻Ly6G⁻F4/80⁺CD11b⁺); inflammatory monocytes (Siglec-F⁻Ly6G⁻F4/80⁺CD11b⁺Ly6C⁺); and Ly6C⁻ monocytes (Siglec-F⁻Ly6G⁻F4/80⁺CD11b⁺Ly6C⁻). Shaded area indicates mean and SEM of total leukocyte frequency. p -values were calculated using Šídák’s multiple-comparisons test. C Serum cytokine levels in WT ( n = 6) and CD5L⁻ ( n = 6) mice. p -values were calculated using the Mann–Whitney test. D Serum RANKL and cross-linked C-telopeptide of type I collagen (CTX-I) levels on day 56 after immunization. p -values were obtained using an unpaired t -test with Welch’s correction. E Splenic gene expression in non-immunized WT ( n = 5) and CD5L⁻ ( n = 5) mice, measured by RT-qPCR and normalized to Actb (β-actin). p -values were calculated using a two-tailed unpaired t -test with Welch’s correction

Article Snippet: C-telopeptide cross-link type I collagen (CTX-I) was measured by an ELISA (IDS Serum CrossLaps, Immunodiagnostic Systems, Boldon, UK) in undiluted samples; detection limit, 0.11 ng/ml.

Techniques: Flow Cytometry, MANN-WHITNEY, Gene Expression, Quantitative RT-PCR, Two Tailed Test